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A novel method of preserving cardiac grafts using a hydrogen-rich water bath

In In vitro studies by CHESS

Exogenously administered hydrogen exerts cytoprotective effects through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms in various disease settings, including organ transplantation. Objective in this study was to evaluate the efficacy of a novel cold storage device equipped with a hydrogen-rich water bath. The study used an established rat heterotopic transplantation model. Syngeneic heart grafts from elderly donors (60- to 70-week-old Lewis rats) …

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Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity

In In vitro studies by CHESS

The ‘colloidal platinum’ stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). Authors evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood fluidity under 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. When applied with …

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Molecular hydrogen regulates the expression of anti-inflammatory proteins in retinal microglia cells

In In vitro studies by CHESS

This study explored the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling …

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Molecular hydrogen exerts a regulating role in the release of pro- and anti-inflammatory cytokines

In In vitro studies by CHESS

Molecular hydrogen (H2) as a new medical gas has an anti-inflammatory effect. In the present study, authors investigated whether heme oxygenase-1 (HO-1) contributes to the anti-inflammatory effect of H2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. RAW 264.7 macrophages were stimulated by LPS (1 μg/mL) with presence or absence of different concentrations of H2. Cell viability and injury were tested by 3-(4,5)-dimethylthiahiazo …

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Molecular hydrogen attenuates fatty acid uptake and lipid accumulation

In In vitro studies by CHESS

There is accumulating evidence that obesity is closely associated with an impaired free fatty acid metabolism as well as with insulin resistance and inflammation. Excessive fatty acid uptake mediated by fatty acid translocase CD36 plays an important role in hepatic steatosis. Molecular hydrogen has been shown to attenuate oxidative stress and improve lipid, glucose and energy metabolism in patients and …

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Hydrogen-dissolved water inhibits growth of human tongue carcinoma cells

In In vitro studies by CHESS

Hydrogen-dissolved water (HD-water) or platinum nanocolloid (Pt-nc) has been individually expected as a new therapeutic agent for oxidative stress-related diseases, whereas little is known about their combined effects on cancer, which were elucidated in the present study. HD-water was prepared by microporous gas bubbling, and supplemented with Pt-nc consisting of 0.003-1 ppm Pt and PVP polymers. Antioxidant activities were examined …

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Hydrogen-rich medium protects human skin fibroblasts from oxidative damage

In In vitro studies by CHESS

Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In the present study, we investigated the protective effects of hydrogen-rich medium on human …

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Radioprotective effect of hydrogen in cultured cells

In In vitro studies by CHESS

It has been demonstrated that hydrogen can selectively reduce hydroxyl and peroxynitrite in vitro. Since most of the ionizing radiation-induced cellular damage is caused by hydroxyl radicals, this study was designed to test the hypothesis that hydrogen may be an effective radioprotective agent. This paper demonstrates that treating cells with hydrogen before irradiation could significantly inhibit ionizing irradiation(IR)-induced Human Lymphocyte …