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Protective effects of hydrogen gas on cell death in Schwann cells

In In vitro studies by CHESS

This study aimed to investigate the protective effects and underlying molecular mechanisms of hydrogen (H2) on high glucose-induced poly (ADP-ribose) polymerase-1 (PARP-1) dependent cell death (PARthanatos) in primary rat Schwann cells. Cultured primary rat Schwann cells were randomly divided into five groups: blank control group (C group), H2 control group (H2 group), high osmotic control group (M group), high glucose …

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Hydrogen-rich water promotes wound healing

In In vitro studies by CHESS

The aim of the present study is to investigate protective effects of hydrogen-rich water (HW) against reactive oxygen species (ROS)-induced cellular harmful events and cell death in human gingival fibroblasts (HGF) and three-dimensional (3D-) gingival tissue equivalents. HW was prepared with a magnesium stick in 600-mL double distilled water (DDW) overnight. Dissolved hydrogen was about 1460 ± 50 μg/L versus approximately 1600 μg/L for …

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Molecular hydrogen inhibits inflammasome activation by targeting mitochondria

In In vitro studies by CHESS

The NLRP3 inflammasome, an intracellular multi-protein complex controlling the maturation of cytokine interleukin-1β, plays an important role in lipopolysaccharide (LPS)-induced inflammatory cascades. Recently, the production of mitochondrial reactive oxygen species (mtROS) in macrophages stimulated with LPS has been suggested to act as a trigger during the process of NLRP3 inflammasome activation that can be blocked by some mitochondria-targeted antioxidants. Known …

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A novel method of preserving cardiac grafts using a hydrogen-rich water bath

In In vitro studies by CHESS

Exogenously administered hydrogen exerts cytoprotective effects through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms in various disease settings, including organ transplantation. Objective in this study was to evaluate the efficacy of a novel cold storage device equipped with a hydrogen-rich water bath. The study used an established rat heterotopic transplantation model. Syngeneic heart grafts from elderly donors (60- to 70-week-old Lewis rats) …

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Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity

In In vitro studies by CHESS

The ‘colloidal platinum’ stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). Authors evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood fluidity under 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. When applied with …

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Molecular hydrogen regulates the expression of anti-inflammatory proteins in retinal microglia cells

In In vitro studies by CHESS

This study explored the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling …

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Molecular hydrogen exerts a regulating role in the release of pro- and anti-inflammatory cytokines

In In vitro studies by CHESS

Molecular hydrogen (H2) as a new medical gas has an anti-inflammatory effect. In the present study, authors investigated whether heme oxygenase-1 (HO-1) contributes to the anti-inflammatory effect of H2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. RAW 264.7 macrophages were stimulated by LPS (1 μg/mL) with presence or absence of different concentrations of H2. Cell viability and injury were tested by 3-(4,5)-dimethylthiahiazo …

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Molecular hydrogen attenuates fatty acid uptake and lipid accumulation

In In vitro studies by CHESS

There is accumulating evidence that obesity is closely associated with an impaired free fatty acid metabolism as well as with insulin resistance and inflammation. Excessive fatty acid uptake mediated by fatty acid translocase CD36 plays an important role in hepatic steatosis. Molecular hydrogen has been shown to attenuate oxidative stress and improve lipid, glucose and energy metabolism in patients and …