The NLRP3 inflammasome, an intracellular multi-protein complex controlling the maturation of cytokine interleukin-1β, plays an important role in lipopolysaccharide (LPS)-induced inflammatory cascades. Recently, the production of mitochondrial reactive oxygen species (mtROS) in macrophages stimulated with LPS has been suggested to act as a trigger during the process of NLRP3 inflammasome activation that can be blocked by some mitochondria-targeted antioxidants. Known …
A novel method of preserving cardiac grafts using a hydrogen-rich water bath
Exogenously administered hydrogen exerts cytoprotective effects through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms in various disease settings, including organ transplantation. Objective in this study was to evaluate the efficacy of a novel cold storage device equipped with a hydrogen-rich water bath. The study used an established rat heterotopic transplantation model. Syngeneic heart grafts from elderly donors (60- to 70-week-old Lewis rats) …
Colloidal platinum in hydrogen-rich water exhibits radical-scavenging activity and improves blood fluidity
The ‘colloidal platinum’ stabilized with polyvinylpyrrolidone (Pt/PVP-colloid) was dispersed in hydrogen-rich water (HW; hydrogen concentration, 0.82 ppm; oxidation-reduction potential, -583 mV) or regular water (RW; <0.01 ppm, +218 mV). Authors evaluated the antioxidant activity of Pt/PVP-colloid in HW or RW on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and improvement of blood fluidity under 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. When applied with …
Neuroprotective effects of water containing molecular hydrogen and Pt nanoparticles
Human brain is the biggest energy consuming tissue in human body. Although it only represents 2% of the body weight, it receives 20% of total body oxygen consumption and 25% of total body glucose utilization. For that reason, brain is considered to be the most vulnerable part of human body against the reactive oxygen species (ROS), a by-product of aerobic …
Molecular hydrogen protects human lymphocyte against heavy ion radiation
The purpose of this study was to investigate the potential protective role of molecular hydrogen (H2) against (12)C(6+) heavy ion radiation, which is a major hazard for space travel and has been also widely used in heavy ion radiotherapy. H2 was dissolved in Roswell Park Memorial Institute (RPMI) 1640 medium under high pressure (0.4 Mpa) to a saturated level. A …
Molecular hydrogen regulates the expression of anti-inflammatory proteins in retinal microglia cells
This study explored the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities. Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling …
Molecular hydrogen exerts a regulating role in the release of pro- and anti-inflammatory cytokines
Molecular hydrogen (H2) as a new medical gas has an anti-inflammatory effect. In the present study, authors investigated whether heme oxygenase-1 (HO-1) contributes to the anti-inflammatory effect of H2 in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. RAW 264.7 macrophages were stimulated by LPS (1 μg/mL) with presence or absence of different concentrations of H2. Cell viability and injury were tested by 3-(4,5)-dimethylthiahiazo …
Molecular hydrogen attenuates fatty acid uptake and lipid accumulation
There is accumulating evidence that obesity is closely associated with an impaired free fatty acid metabolism as well as with insulin resistance and inflammation. Excessive fatty acid uptake mediated by fatty acid translocase CD36 plays an important role in hepatic steatosis. Molecular hydrogen has been shown to attenuate oxidative stress and improve lipid, glucose and energy metabolism in patients and …
Hydrogen-dissolved water inhibits growth of human tongue carcinoma cells
Hydrogen-dissolved water (HD-water) or platinum nanocolloid (Pt-nc) has been individually expected as a new therapeutic agent for oxidative stress-related diseases, whereas little is known about their combined effects on cancer, which were elucidated in the present study. HD-water was prepared by microporous gas bubbling, and supplemented with Pt-nc consisting of 0.003-1 ppm Pt and PVP polymers. Antioxidant activities were examined …
Hydrogen-rich medium protects human skin fibroblasts from oxidative damage
Reactive oxygen species (ROS) are an important factor in the development of skin lesions in diabetes. A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. Fibroblasts are a key component of skin. In the present study, we investigated the protective effects of hydrogen-rich medium on human …