Neuroprotective effects of water containing molecular hydrogen and Pt nanoparticles

In In vitro studiesby CHESS

Human brain is the biggest energy consuming tissue in human body. Although it only represents 2% of the body weight, it receives 20% of total body oxygen consumption and 25% of total body glucose utilization. For that reason, brain is considered to be the most vulnerable part of human body against the reactive oxygen species (ROS), a by-product of aerobic respiration. Oxidative stress is directly related to a series of brain dysfunctional disease such as Alzheimer’s disease, Parkinson’s disease etc. Electrolyzed reduced water (ERW) is a functional drinking water containing a lot of molecular hydrogen and a small amount of platinum nanoparticles (Pt NPs). ERW is known to scavenge ROS and protect DNA from oxidative damage. Authors previously showed that ERW was capable of extending lifespan of Caenorhabditis elegans by scavenging ROS. Molecular hydrogen could scavenge ROS and protected brain from oxidative stress. Pt NPs are also a new type of multi-functional ROS scavenger. In this research, authors used TI-200S ERW derived from 2 mM NaOH solution produced by a batch type electrolysis device and model waters containing molecular hydrogen and synthetic Pt NPs of 2-3 nm sizes as research models of ERW to examine the anti-oxidant capabilities of ERW on several kinds of neural cells such as PC12, N1E115, and serum free mouse embryo (SFME) cells. Authors pretreated the ERW and 200 μM H2O2 and examined the neuroprotective effects of ERW on PC12, N1E115 and SFME cells, using WST-8 method. Authors also examined the intracellular ROS scavenging effects of ERW on N1E115 cells after pretreated cells with ERW and H2O2 using DCFH-DA. We checked the protective effects of ERW on mitochondria and cytoplasm by Rh123 and Fuo-3 AM stain. We also examined the ATP production of SFME cells after pretreated with ERW and H2O2 by Bioluminescence Assay Kit. Finally, we used dissolved hydrogen (DH) and Pt NPs as research models to examine their neuroprotective effects. ERW significantly reduced the cell death induced by H2O2 pretreatment. ERW also scavenged the intracellular ROS and prevented the decrease of mitochondrial membrane potential and ATP production induced by ROS. We also examined the neuroprotective effects of molecular hydrogen and Pt NPs and showed that both molecular hydrogen and Pt NPs contributed to the neuroprotective effects of ERW. The results suggest that ERW is beneficial for the prevention and alleviation of oxidative stress-induced human neurodegenerative diseases.

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Yan H, Kashiwaki T, Hamasaki T, et al. The neuroprotective effects of electrolyzed reduced water and its model water containing molecular hydrogen and Pt nanoparticles. BMC Proc. 2011 Nov 22;5 Suppl 8:P69.