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Amelioration of retinitis pigmentosa by hydrogen-rich saline

In Animal studies, Eye / Ear by CHESS

The main aim of this study was to investigate the effects of hydrogen-rich saline (HRS) on microglia activation and Sirtuin type 1 (Sirt1) in rats with N-methyl-N-nitrosourea (MNU)-induced retinitis pigmentosa (RP). Rats were divided into norm (N) group, model (M) group and HRS (H) group. Rats in M and H groups were given saline and HRS respectively prior to and …

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Prevention of hearing loss by intravenous administration of hydrogen-rich saline

In Animal studies, Eye / Ear by CHESS

Hydrogen-rich water, which is a potent antioxidant agent, was investigated for its protective effects against ischemic damage of the cochlea in gerbils. The animals were subjected to transient cochlear ischemia by occluding the bilateral vertebral arteries for 15min. Five milliliters of hydrogen-rich saline was then intravenously administered immediately after the insult. Saline without hydrogen was used as a control. Effects …

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Prevention of ischemia-induced hearing loss by hydrogen-rich saline

In Animal studies, Eye / Ear by CHESS

Hydrogen-rich water, which is a potent antioxidant agent, was investigated for its protective effects against ischemic damage of the cochlea in gerbils. The animals were subjected to transient cochlear ischemia by occluding the bilateral vertebral arteries for l5min. Five milliliters of hydrogen-rich saline was then intravenously administered immediately after the insult. Saline without hydrogen was used as a control. Effects …

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Hydrogen prevents corneal endothelial damage

In Animal studies, Eye / Ear by CHESS

In phacoemulsification, ultrasound induces hydroxyl radical (·OH) formation, damaging corneal endothelium. Whether H2 can prevent such oxidative damage in phacoemulsification was examined by in vitro and in vivo studies. H2 was dissolved in a commercial irrigating solution. The effects of H2 against ·OH generation were first confirmed in vitro by electron-spin resonance (ESR) and hydroxyphenyl fluorescein (HPF). ESR showed a …

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Inhaled hydrogen promotes survival of retinal cells

In Animal studies, Eye / Ear by CHESS

Retinal ischemia/reperfusion (I/R) injury plays a crucial role in the pathophysiology of various ocular diseases. Intraperitoneal injection or ocular instillation with hydrogen (H2)-rich saline was recently shown to be neuroprotective in the retina due to its anti-oxidative and anti-inflammatory effects. This study aims to explore whether postconditioning with inhaled H2 can protect retinal ganglion cells (RGCs) in a rat model …

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Hydrogen is ineffective in O2-induced retinopathy

In Animal studies, Eye / Ear by CHESS

Hydrogen-rich saline (HRS) is a novel protection against various oxidative disorders and almost all types of inflammation. Moreover, its toxicity and side effects are rarely reported. The authors sought to clarify the protective effect of HRS against the oxygen-induced retinopathy (OIR) in C57BL/6 J model. The OIR in the HRS treated mice and the untreated controls were systematically compared. The …

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Protective effects of hydrogen against photoreceptor degeneration

In Animal studies, Eye / Ear by CHESS

The N-methyl-N-nitrosourea (MNU)-treated rat is typically used as an animal model of chemically-induced retinitis pigmentosa (RP). Reactive oxygen species (ROS) have been recognized as the crucial contributor to the retinal photoreceptor apoptosis seen in MNU-treated rats. In the present study, authors explored the therapeutic effects of hydrogen-rich saline (HRS), a selective ROS scavenger, on MNU-induced photoreceptor degeneration. Intraperitoneal (IP) administration …

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Inhaled hydrogen gas therapy for prevention of noise-induced hearing loss

In Animal studies, Eye / Ear by CHESS

Reactive oxygen species (ROS) that form in the inner ear play an important role in noise-induced hearing loss (NIHL). Recent studies have revealed that molecular hydrogen (H2) has great potential for reducing ROS. In this study, we examined the potential of hydrogen gas to protect against NIHL. The authors tested this hypothesis in guinea pigs with 0.5%, 1.0% and 1.5% …

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Hydrogen-rich saline has a neuroprotective effect on retinal ischemia-reperfusion injury

In Animal studies, Eye / Ear by CHESS

Overactivation of poly (ADP-ribose) polymerase 1 (PARP-1), as a result of sustained DNA oxidation in ischemia-reperfusion injury, triggers programmed cell necrosis and apoptosis. The present study was conducted to demonstrate whether hydrogen-rich saline (HRS) has a neuroprotective effect on retinal ischemia reperfusion (RIR) injury through inhibition of PARP-1 activation. RIR was induced by transient elevation of intraocular pressure in rats. …

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Hydrogen-rich saline provides protection and treatment against light-induced retinal damage

In Animal studies, Eye / Ear by CHESS

Oxidative reactions are thought to be a major cause of light-induced retinal degeneration. This study was designed to investigate the effects of hydrogen-rich saline (HRS) on the prevention and treatment of light-induced retinal injury in rats. Male Sprague-Dawley rats were divided randomly into three groups: light damage, HRS prevention (5 ml/kg, 30 min before intensive light exposure), and HRS treatment (5 ml/kg per …