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Lung inflation with hydrogen reduced lung injury

In Animal studies, Lung by CHESS

Lung ischemia-reperfusion injury (IRI) may be attenuated through carbon monoxide (CO)’s anti-inflammatory effect or hydrogen (H2)’s anti-oxidant effect. In this study, the effects of lung inflation with CO, H2, or both during the cold ischemia phase on graft function were observed. Rat donor lungs, inflated with 40% oxygen (control group), 500ppm CO (CO group), 3% H2 (H2 group) or 500ppm …

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Gaseous hydrogen improves myocardial dysfunction

In Animal studies, Cardiovascular by CHESS

Immediate inhalation of hydrogen (H2) has been demonstrated to improve post-resuscitation (PR) myocardial dysfunction. In this study, the authors investigated the effects of delayed treatments with H2 on myocardial dysfunction in a porcine model of cardiac arrest (CA). Twenty male pigs weighing 39 ± 2 kg were utilized. Ventricular fibrillation was electrically induced and untreated for 10 min. All the …

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Hydrogen inhalation improves survival in hemorrhagic shock

In Animal studies, Cardiovascular by CHESS

Hemorrhagic shock is a life-threatening condition. In addition to conventional treatments such as hemostasis and fluid resuscitation, further investigations are warranted to develop more effective treatments. The study investigated the effectiveness of hydrogen (H2) gas inhalation in a fixed-pressure model of hemorrhagic shock in rats. Hemorrhagic shock is induced by withdrawing blood from the left carotid artery until the mean …

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Inhaled hydrogen ameliorates bowel dysfunction

In Animal studies, Gut by CHESS

Gastrointestinal dysmotility frequently occurs during sepsis and multiple organ failure, remaining a major cause of morbidity and mortality in critically ill patients. Previous studies have shown that hydrogen, a new therapeutic gas, can improve various organ damage associated with sepsis. In this study, the authors investigated the protective efficacy of inhaled hydrogen against lipopolysaccharide (LPS)-induced ileus. Sepsis was induced in …

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Hydrogen prevents corneal endothelial damage

In Animal studies, Eye / Ear by CHESS

In phacoemulsification, ultrasound induces hydroxyl radical (·OH) formation, damaging corneal endothelium. Whether H2 can prevent such oxidative damage in phacoemulsification was examined by in vitro and in vivo studies. H2 was dissolved in a commercial irrigating solution. The effects of H2 against ·OH generation were first confirmed in vitro by electron-spin resonance (ESR) and hydroxyphenyl fluorescein (HPF). ESR showed a …

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Molecular hydrogen modifies free radical-dependent generation of oxidized phospholipid mediators

In In vitro studies by CHESS

The authors previously showed that H2 acts as a novel antioxidant to protect cells against oxidative stress. Subsequently, numerous studies have indicated the potential applications of H2 in therapeutic and preventive medicine. Moreover, H2 regulates various signal transduction pathways and the expression of many genes. However, the primary targets of H2 in the signal transduction pathways are unknown. Here, the …

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Hydrogen as a therapeutic strategy against ocular diseases

In Review papers by CHESS

Hydrogen, one of the most well-known natural molecules, has been used in numerous medical applications owing to its ability to selectively neutralize cytotoxic reactive oxygen species and ameliorate hazardous inflammations. Hydrogen can exert protective effects on various reactive oxygen species-related diseases, including the transplantation-induced intestinal graft injury, chronic inflammation, ischemia-reperfusion injuries, and so on. Especially in the eye, hydrogen has …

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Preventive effect of hydrogen water on bladder dysfunction

In Animal studies, Kidney by CHESS

Bladder ischemia and oxidative stress contribute to the pathogenesis of bladder dysfunction caused by bladder outlet obstruction. H2 reportedly acts as an effective antioxidant. The authors investigated whether oral ingestion of H2 water would have a beneficial effect on bladder function in a rat model of bladder outlet obstruction. H2 water was made by dissolving H2 gas in ordinary drinking …

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Molecular hydrogen alleviates motor deficits and muscle degeneration in Duchenne dystrophy

In Animal studies, Muscle by CHESS

Duchenne muscular dystrophy (DMD) is a devastating muscle disease caused by a mutation in DMD encoding dystrophin. Oxidative stress accounts for dystrophic muscle pathologies in DMD. The authors examined the effects of molecular hydrogen in mdx mice, a model animal for DMD. The pregnant mother started to take supersaturated hydrogen water (>5 ppm) ad libitum from E15.5 up to weaning of …

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Hydrogen ameliorates hepatic reperfusion injury

In Animal studies, Liver by CHESS

Hydrogen gas reduces ischemia and reperfusion injury (IRI) in the liver and other organs. However, the precise mechanism remains elusive. We investigated whether hydrogen gas ameliorated hepatic I/R injury after cold preservation. Rat liver was subjected to 48-h cold storage in University of Wisconsin solution. The graft was reperfused with oxygenated buffer with or without hydrogen at 37° for 90 …